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Research article

References

  1. Antunes LCS, Impeeri F, Carattoli A, Visca P. 2011. Deciphering the multifactorial nature of Acinetobacter baumannii pathogenicity. PLoS ONE 6: e22674.
    Pubmed PMC CrossRef
  2. Chuang YC, Sheng WH, Li SY, Lin YC, Wang JT, Chen YC, Chang SC. 2011. Influence of genospecies of Acinetobacter baumannii complex on clinical outcomes of patients with Acinetobacter bacteremia. Clin. Infect. Dis. 52: 352-360.
    Pubmed CrossRef
  3. Chusri S, Chongsuvivatwong V, Rivera JI, Slipapojakul K, Singkhamana K, McNeil E, Doi Y. 2014. Clinical outcomes of hospital-acquired infection with Acinetobacter nosocomialis and Acinetobacter pittii. Antimicrob. Agents Chemother. 58:4172-4179.
    Pubmed PMC CrossRef
  4. Clemmer KM, Bonomo RA, Rather PN. 2011. Genetic analysis of surface motility in Acinetobacter baumannii. Microbiology 157: 2534-2544.
    Pubmed PMC CrossRef
  5. de Breij A, Dijkshoorn L, Lagendijk E, van der Meer J, Koster A, Bloemberg G, et al. 2010. Do biofilm formation and interactions with human cells explain the clinical success of Acinetobacter baumannii? PLoS One 5: e10732.
    Pubmed PMC CrossRef
  6. Dijkshoorn L, Nemec A, Seifert H. 2007. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 5: 939-951.
    Pubmed CrossRef
  7. Eijkelkamp BA, Stroeher UH, Hassan KA, Papadimitrious MS, Paulsen IT, Brown MH. 2011. Adherence and motility characteristics of clinical Acinetobacter baumannii isolates. FEMS Microbiol. Lett. 323: 44-51.
    Pubmed CrossRef
  8. Fishbain J, Peleg AY. 2010. Treatment of Acinetobacter infections. Clin. Infect. Dis. 51: 79-84.
    Pubmed CrossRef
  9. Fitzpatrick MA, Ozer E, Bolon MK, Hauser AR. 2015. Influence of ACB complex genospecies on clinical outcomes in a US hospital with high rates of multidrug resistance. J. Infect. 70: 144-152.
    Pubmed PMC CrossRef
  10. Foster TJ. 2005. Immune evasion by staphylococci. Nat. Rev. Microbiol. 3: 948-958.
    Pubmed CrossRef
  11. Jefferson KK. 2004. What drives bacteria to produce a biofilm? FEMS Microbiol. Lett. 236: 163-173.
    Pubmed CrossRef
  12. Kim SW, Choi CH, Moon DC, Jin JS, Lee JH, Shin JH, et al. 2009. Serum resistance of Acinetobacter baumannii through the binding of factor H to outer membrane proteins. FEMS Microbiol. Lett. 301: 224-231.
    Pubmed CrossRef
  13. Ko KS, Suh JY, Kwon KT, Jung SI, Park KH, Kang CI, et al. 2007. High rates of resistance to colistin and polymyxin B in subgroups of Acinetobacter baumannii isolates from Korea. J. Antimicrob. Chemother. 60: 1163-1167.
    Pubmed CrossRef
  14. Liao CH, Sheng WH, Chen YC, Hung CC, Wang JT, Chang SC. 2007. Predictive value of the serum bactericidal test for mortality in patients infected with multidrug-resistant Acinetobacter baumannii. J. Infect. 55: 149-157.
    Pubmed CrossRef
  15. Lee HW, Koh YM, Kim J, Lee J, Lee YC, Seol SY, Cho DT. 2008. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clin. Microbiol. Infect. 14: 49-54.
    Pubmed CrossRef
  16. March C, Regueiro V, Llobet E, Moranta D, Morey P, Garmendia J, Bengoechea JA. 2010. Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. PLoS One 5: e10033.
    Pubmed PMC CrossRef
  17. McConnell MJ, Actis L, Pachón J. 2013. Acinetobacter baumannii:human infections, factors contributing to pathogenesis and animal models. FEMS Microbiol. Rev. 37: 130-135.
    Pubmed CrossRef
  18. Mussi MA, Gaddy JA, Cabruja M, Arivett BA, Viale AM, Rasia R, Actis LA. 2010. The opportunistic human pathogen Acinetobacter baumannii senses and responds to light. J. Bacteriol. 192: 6226-6345.
    Pubmed PMC CrossRef
  19. Nemec A, Krizova L, Maixnerova M, Sedo O, Brisse S, Higgins PG. 2015. Acinetobacter seifertii sp. nov. a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. Int. J. Syst. Evol. Microbiol. 65: 934-942.
    Pubmed CrossRef
  20. Park KH, Shin JH, Lee SY, Kim SH, Jang MO, Kang SJ, et al. 2013. The clinical characteristics, carbapenem resistance, and outcome of Acinetobacter baumannii bacteremia according to genospecies. PLoS One 8: e65026.
    Pubmed PMC CrossRef
  21. Park YK, Choi JY, Jung SI, Park KH, Lee H, Jung DS, et al. 2009. Two distinct clones of carbapenem-resistant Acinetobacter baumannii isolates from Korean hospitals. Diagn. Microbiol. Infect. Dis. 64: 389-395.
    Pubmed CrossRef
  22. Park YK, Jung SI, Park KH, Kim DH, Choi JY, Kim SH, Ko KS. 2012. Changes in antimicrobial susceptibility and major clones of Acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in Korea over 7 years. J. Med. Microbiol. 61: 71-79.
    Pubmed CrossRef
  23. Peleg AY, de Breij A, Adams MD, Cerqueira GM, Mocali S, Galardini M, et al. 2012. The success of Acinetobacter species;genetic, metabolic and virulence attributes. PLoS One 7: e46984.
    Pubmed PMC CrossRef
  24. Rodriguez-Bano J, Marti S, Soto S, Fernandez-Cuenca F, Cisneros JM, Pachon J, et al. 2008. Biofilm formation in Acinetobacter baumannii: associated features and clinical implications. Clin. Microbiol. Infect. 14: 276-278.
    Pubmed CrossRef
  25. Seidi K, Goerke C, Wolz C, Mack D, Berger-Bächi B, Bischoff M. 2008. Staphylococcus aureus CcpA affects biofilm formation. Infect. Immun. 76: 2044-2050.
    Pubmed PMC CrossRef
  26. Shamim S, Rehman A, Qazi MH. 2014. Swimming, swarming, twitching, and chemotactic responses of Culpriavidus metallidurans CH34 and Pseudomonas putida mt2 in the presence of cadmium. Arch. Environ. Contam. Toxicol. 66: 407-414.
    Pubmed CrossRef
  27. Touchon M, Cury J, Yoon EJ, Krizova L, Cerqueira GC, Murphy C, et al. 2014. The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences. Genome Biol. Evol. 6: 2866-2882.
    Pubmed PMC CrossRef
  28. Towner KJ. 2009. Acinetobacter: an old friend, but a new enemy. J. Infect. 73: 355-363.
    CrossRef
  29. Wang J, Ruan Z, Feng Y, Fu Y, Jiang Y, Wang H, Yu Y. 2014. Species distribution of clinical Acinetobacter isolates revealed by different identification techniques. PLoS One 9: e104882.
    Pubmed PMC CrossRef

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Article

Research article

J. Microbiol. Biotechnol. 2016; 26(1): 171-179

Published online January 28, 2016 https://doi.org/10.4014/jmb.1507.07076

Copyright © The Korean Society for Microbiology and Biotechnology.

Comparison of the Virulence-Associated Phenotypes of Five Species of Acinetobacter baumannii Complex

In Young Na 1, Eun Seon Chung 1, Chang-Yun Jung 1, Dae Hun Kim 1, Juyoun Shin 1, KyeongJin Kang 2, Seong-Tae Kim 1 and Kwan Soo Ko 1*

1Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea, 2Department of Anatomy and Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea

Received: July 21, 2015; Accepted: October 2, 2015

Abstract

In this study, we compared the virulence-associated factors of Acinetobacter baumannii complex
species. Sixty-three isolates of five A. baumannii complex species, including 19 A. baumannii, 15
A. nosocomialis, 13 A. seifertii, 13 A. pittii, and 3 A. calcoaceticus isolates, were included in this
study. For all isolates, biofilm formation, A549 cell adherence, resistance to normal human
serum, and motility were evaluated. A. baumannii complex isolates showed diversity in biofilm
formation, A549 cell adherence, and serum resistance, and no strong positive relationships
among these virulence characteristics. However, A. seifertii showed relatively consistent
virulence-associated phenotypes. In addition, A. baumannii clone ST110 exhibited consistently
high virulence-associated phenotypes. Motility was observed in seven isolates, and all four
A. baumannii ST110 isolates showed twitching motility. Although some inconsistencies in
virulence-associated phenotypes were seen, high virulence characteristics were observed in
A. seifertii, which has been mainly reported in Korea and shows high rates of colistin
resistance.

Keywords: Acinetobacter seifertii, twitching, serum resistance, A549 adherence

References

  1. Antunes LCS, Impeeri F, Carattoli A, Visca P. 2011. Deciphering the multifactorial nature of Acinetobacter baumannii pathogenicity. PLoS ONE 6: e22674.
    Pubmed KoreaMed CrossRef
  2. Chuang YC, Sheng WH, Li SY, Lin YC, Wang JT, Chen YC, Chang SC. 2011. Influence of genospecies of Acinetobacter baumannii complex on clinical outcomes of patients with Acinetobacter bacteremia. Clin. Infect. Dis. 52: 352-360.
    Pubmed CrossRef
  3. Chusri S, Chongsuvivatwong V, Rivera JI, Slipapojakul K, Singkhamana K, McNeil E, Doi Y. 2014. Clinical outcomes of hospital-acquired infection with Acinetobacter nosocomialis and Acinetobacter pittii. Antimicrob. Agents Chemother. 58:4172-4179.
    Pubmed KoreaMed CrossRef
  4. Clemmer KM, Bonomo RA, Rather PN. 2011. Genetic analysis of surface motility in Acinetobacter baumannii. Microbiology 157: 2534-2544.
    Pubmed KoreaMed CrossRef
  5. de Breij A, Dijkshoorn L, Lagendijk E, van der Meer J, Koster A, Bloemberg G, et al. 2010. Do biofilm formation and interactions with human cells explain the clinical success of Acinetobacter baumannii? PLoS One 5: e10732.
    Pubmed KoreaMed CrossRef
  6. Dijkshoorn L, Nemec A, Seifert H. 2007. An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat. Rev. Microbiol. 5: 939-951.
    Pubmed CrossRef
  7. Eijkelkamp BA, Stroeher UH, Hassan KA, Papadimitrious MS, Paulsen IT, Brown MH. 2011. Adherence and motility characteristics of clinical Acinetobacter baumannii isolates. FEMS Microbiol. Lett. 323: 44-51.
    Pubmed CrossRef
  8. Fishbain J, Peleg AY. 2010. Treatment of Acinetobacter infections. Clin. Infect. Dis. 51: 79-84.
    Pubmed CrossRef
  9. Fitzpatrick MA, Ozer E, Bolon MK, Hauser AR. 2015. Influence of ACB complex genospecies on clinical outcomes in a US hospital with high rates of multidrug resistance. J. Infect. 70: 144-152.
    Pubmed KoreaMed CrossRef
  10. Foster TJ. 2005. Immune evasion by staphylococci. Nat. Rev. Microbiol. 3: 948-958.
    Pubmed CrossRef
  11. Jefferson KK. 2004. What drives bacteria to produce a biofilm? FEMS Microbiol. Lett. 236: 163-173.
    Pubmed CrossRef
  12. Kim SW, Choi CH, Moon DC, Jin JS, Lee JH, Shin JH, et al. 2009. Serum resistance of Acinetobacter baumannii through the binding of factor H to outer membrane proteins. FEMS Microbiol. Lett. 301: 224-231.
    Pubmed CrossRef
  13. Ko KS, Suh JY, Kwon KT, Jung SI, Park KH, Kang CI, et al. 2007. High rates of resistance to colistin and polymyxin B in subgroups of Acinetobacter baumannii isolates from Korea. J. Antimicrob. Chemother. 60: 1163-1167.
    Pubmed CrossRef
  14. Liao CH, Sheng WH, Chen YC, Hung CC, Wang JT, Chang SC. 2007. Predictive value of the serum bactericidal test for mortality in patients infected with multidrug-resistant Acinetobacter baumannii. J. Infect. 55: 149-157.
    Pubmed CrossRef
  15. Lee HW, Koh YM, Kim J, Lee J, Lee YC, Seol SY, Cho DT. 2008. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clin. Microbiol. Infect. 14: 49-54.
    Pubmed CrossRef
  16. March C, Regueiro V, Llobet E, Moranta D, Morey P, Garmendia J, Bengoechea JA. 2010. Dissection of host cell signal transduction during Acinetobacter baumannii-triggered inflammatory response. PLoS One 5: e10033.
    Pubmed KoreaMed CrossRef
  17. McConnell MJ, Actis L, Pachón J. 2013. Acinetobacter baumannii:human infections, factors contributing to pathogenesis and animal models. FEMS Microbiol. Rev. 37: 130-135.
    Pubmed CrossRef
  18. Mussi MA, Gaddy JA, Cabruja M, Arivett BA, Viale AM, Rasia R, Actis LA. 2010. The opportunistic human pathogen Acinetobacter baumannii senses and responds to light. J. Bacteriol. 192: 6226-6345.
    Pubmed KoreaMed CrossRef
  19. Nemec A, Krizova L, Maixnerova M, Sedo O, Brisse S, Higgins PG. 2015. Acinetobacter seifertii sp. nov. a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. Int. J. Syst. Evol. Microbiol. 65: 934-942.
    Pubmed CrossRef
  20. Park KH, Shin JH, Lee SY, Kim SH, Jang MO, Kang SJ, et al. 2013. The clinical characteristics, carbapenem resistance, and outcome of Acinetobacter baumannii bacteremia according to genospecies. PLoS One 8: e65026.
    Pubmed KoreaMed CrossRef
  21. Park YK, Choi JY, Jung SI, Park KH, Lee H, Jung DS, et al. 2009. Two distinct clones of carbapenem-resistant Acinetobacter baumannii isolates from Korean hospitals. Diagn. Microbiol. Infect. Dis. 64: 389-395.
    Pubmed CrossRef
  22. Park YK, Jung SI, Park KH, Kim DH, Choi JY, Kim SH, Ko KS. 2012. Changes in antimicrobial susceptibility and major clones of Acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in Korea over 7 years. J. Med. Microbiol. 61: 71-79.
    Pubmed CrossRef
  23. Peleg AY, de Breij A, Adams MD, Cerqueira GM, Mocali S, Galardini M, et al. 2012. The success of Acinetobacter species;genetic, metabolic and virulence attributes. PLoS One 7: e46984.
    Pubmed KoreaMed CrossRef
  24. Rodriguez-Bano J, Marti S, Soto S, Fernandez-Cuenca F, Cisneros JM, Pachon J, et al. 2008. Biofilm formation in Acinetobacter baumannii: associated features and clinical implications. Clin. Microbiol. Infect. 14: 276-278.
    Pubmed CrossRef
  25. Seidi K, Goerke C, Wolz C, Mack D, Berger-Bächi B, Bischoff M. 2008. Staphylococcus aureus CcpA affects biofilm formation. Infect. Immun. 76: 2044-2050.
    Pubmed KoreaMed CrossRef
  26. Shamim S, Rehman A, Qazi MH. 2014. Swimming, swarming, twitching, and chemotactic responses of Culpriavidus metallidurans CH34 and Pseudomonas putida mt2 in the presence of cadmium. Arch. Environ. Contam. Toxicol. 66: 407-414.
    Pubmed CrossRef
  27. Touchon M, Cury J, Yoon EJ, Krizova L, Cerqueira GC, Murphy C, et al. 2014. The genomic diversification of the whole Acinetobacter genus: origins, mechanisms, and consequences. Genome Biol. Evol. 6: 2866-2882.
    Pubmed KoreaMed CrossRef
  28. Towner KJ. 2009. Acinetobacter: an old friend, but a new enemy. J. Infect. 73: 355-363.
    CrossRef
  29. Wang J, Ruan Z, Feng Y, Fu Y, Jiang Y, Wang H, Yu Y. 2014. Species distribution of clinical Acinetobacter isolates revealed by different identification techniques. PLoS One 9: e104882.
    Pubmed KoreaMed CrossRef