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Research article

Article

Research article

J. Microbiol. Biotechnol. 2015; 25(11): 1871-1879

Published online November 28, 2015 https://doi.org/10.4014/jmb.1504.04080

Copyright © The Korean Society for Microbiology and Biotechnology.

Role of flgA for Flagellar Biosynthesis and Biofilm Formation of Campylobacter jejuni NCTC11168

Joo-Sung Kim 1, 2*, Changwon Park 1 and Yun-Ji Kim 1, 2

1Korea Food Research Institute, Seongnam 463-746, Republic of Korea, 2Department of Food Biotechnology, Korea University of Science and Technology, Daejeon 305-333, Republic of Korea

Received: April 29, 2015; Accepted: July 22, 2015

Abstract

The complex roles of flagella in the pathogenesis of Campylobacter jejuni, a major cause of
worldwide foodborne diarrheal disease, are important. Compared with the wild-type, an
insertional mutation of the flgA gene (cj0769c) demonstrated significant decrease in the biofilm
formation of C. jejuni NCTC11168 on major food contact surfaces, such as polystyrene,
stainless steel, and borosilicate glass. The flgA mutant was completely devoid of flagella and
non-motile whereas the wild-type displayed the full-length flagella and motility. In addition,
the biofilm formation of the wild-type was inversely dependent on the viscosity of the media.
These results support that flagellar-mediated motility plays a significant role in the biofilm
formation of C. jejuni NCTC11168. Moreover, our adhesion assay suggests that it plays an
important role during biofilm maturation after initial attachment. Furthermore, C. jejuni
NCTC11168 wild-type formed biofilm with a net-like structure of extracellular fiber-like
material, but such a structure was significantly reduced in the biofilm of the flgA mutant. It
supports that the extracellular fiber-like material may play a significant role in the biofilm
formation of C. jejuni. This study demonstrated that flgA is essential for flagellar biosynthesis
and motility, and plays a significant role in the biofilm formation of C. jejuni NCTC11168.

Keywords: Campylobacter jejuni, flgA, biofilm, flagella, motility, extracellular fiber-like material