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Research article

Food Microbiology and Biotechnology

Effects of Oral Intake of Kimchi-Derived Lactobacillus plantarum K8 Lysates on Skin Moisturizing

Hangeun Kim 1, Hye Rim Kim 2, Bong jun Jeong 2, Seung Su Lee 3, Tae-Rahk Kim 1, 3, Ji Hye Jeong 3, Miyeong Lee 3, Sinai Lee 3, Jong Suk Lee 4 and Dae Kyun Chung 1, 2, 3*

1RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Republic of Korea, 2Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, 3Skin Biotechnology Center, Gyeonggi Biocenter, Suwon 443-766, Republic of Korea, 4Gyeonggi Institute of Science & Technology Promotion, Suwon 443-270, Republic of Korea

Received: July 28, 2014; Accepted: August 28, 2014

J. Microbiol. Biotechnol. 2015; 25(1): 74-80

Published January 28, 2015 https://doi.org/10.4014/jmb.1407.07078

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

Skin is the soft outer covering of vertebrates that provides protection from pathogenic infection, physical damage, or UV irradiation, and controls body temperature and water content. In this study, we examined the effects of oral intake of kimchi-derived Lactobacillus plantarum K8 lysates on skin moisturizing. In an in vitro study, we observed that the hyaluronic acid content increased in HaCaT cells treated with L. plantarum K8 lysates. Oral administration of L. plantarum K8 lysates effectively attenuated the horny layer formation and decreased epidermal thickening in DNCB-treated SKH-1 hairless mice skin. The damage to barrier function was reduced after 8 weeks of oral administration of L. plantarum K8 lysates as compared with that in the atopic dermatitis mice. For the test with volunteers, we manufactured experimental candy containing 2.1% L. plantarum K8 lysates, while control candy did not contain bacterial lysate. A significant increase in hydration in the experimental candy-administered group as compared with the control candy-administered group was observed on the face after 4 and 8 weeks, and on the forearm after 4 weeks. Decreases in horny layer thickness and TEWL value were observed on the face and forearm of the experimental group. Together, the in vitro cell line and in vivo mouse studies revealed that L. plantarum K8 lysates have a moisturizing effect. A clinical research study with healthy volunteers also showed an improvement in barrier repair and function when volunteers took L. plantarum K8 lysates-containing candy. Thus, our results suggest that L. plantarum K8 lysates may help to improve skin barrier function.

Keywords

Lactobacillus plantarum, L. plantarum K8-LTA, bacterial lysates, atopic dermatitis, aglycone isoflavone, moisturizer

References

  1. Anderson P C, D inulos JG. 2 009. A re t h e new moisturizers more effective? Curr. Opin. Pediatr. 21: 486-490.
    Pubmed CrossRef
  2. Beeckman D, Schoonhoven L, Verhaeghe S, Heyneman A, Defloor T. 2009. Prevention and treatment of incontinenceassociated dermatitis: literature review. J. Adv. Nurs. 65:1141-1154.
    Pubmed CrossRef
  3. Chan LS. 2008. Atopic dermatitis in 2008. Curr. Dir. Autoimmun. 10: 76-118.
    Pubmed CrossRef
  4. Cooper KD. 1994. Atopic dermatitis: recent trends in pathogenesis and therapy. J. Invest. Dermatol. 102: 128-137.
    Pubmed CrossRef
  5. Flynn TC, Petros J, Clark RE, Viehman GE. 2001. Dry skin and moisturizers. Clin. Dermatol. 19: 387-392.
    CrossRef
  6. Georgieva RN, IIiev IN, Chipeva VA, Dimitonova SP, Samelis J, Danova ST. 2008. Identification and in vitro characterization of Lactobacillus plantarum strains from artisanal Bulgarian white brined cheeses. J. Basic Microbiol. 48: 234-244.
    Pubmed CrossRef
  7. Ginsburg I. 2002. Role of lipoteichoic acid in infection and inflammation. Lancet Infect. 2: 171-179.
    CrossRef
  8. Hannuksela A, Kinnunen T. 1992. Moisturizers prevent irritant dermatitis. Acta Derm. Venereol. 72: 42-44.
    Pubmed
  9. Kwon HK, Lee CG, So JS, Chae CS, Hwang JS, Sahoo A, et al. 2010. Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders. Proc. Natl. Acad. Sci. USA 107: 2159-2164.
    Pubmed PMC CrossRef
  10. McCusker MM, Grant-Kels JM. 2010. Healing fats of the skin: the structural and immunologic roles of the omega-6 and omega-3 fatty acids. Clin. Dermatol. 28: 440-451.
    Pubmed CrossRef
  11. Meydani SN, Ha WK. 2000. Immunologic effects of yogurt. Am. J. Clin. Nutr. 71: 861-872.
    Pubmed
  12. Ozdemir O. 2010. Various effects of different probiotic strains in allergic disorders: an update from laboratory and clinical data. Clin. Exp. Immunol. 160: 295-304.
    Pubmed PMC CrossRef
  13. Saijic D, Asiniwasis R, Skotnicki-Grant S. 2012. A look at epidermal barrier function in atopic dermatitis: physiologic lipid replacement and the role of ceramide. Skin Therapy Lett. 7: 6-9.
  14. Seo HS, Michalek SM, Nahm MH. 2008. Lipoteichoic acid is important in innate immune responses to gram-positive bacteria. Infect. Immunol. 76: 206-213.
    Pubmed PMC CrossRef
  15. Simpson E, Böhling A, Bielfeldt S, Bosc C, Kerrouche N. 2013. Improvement of skin barrier function in atopic dermatitis patients with a new moisturizer containing a ceramide precursor. J. Dermatol. Treat. 24: 122-125.
    Pubmed CrossRef
  16. Snapper CM, Paul WE. 1987. Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate Ig isotype production. Science 236: 944-947.
    Pubmed CrossRef
  17. Varothai S, Nitayavardhana S, Kulthanan K. 2013. Moisturizers for patients with atopic dermatitis. Asian Pac. J. Allergy Immunol. 31: 91-98.
    Pubmed
  18. Wallace TD, Bradley S, Buckley ND, Green-Johnson JM. 2003. Interactions of lactic acid bacteria with human intestinal epithelial cells: effects on cytokine production. J. Food Protect. 66: 466-472.
    Pubmed

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Article

Research article

J. Microbiol. Biotechnol. 2015; 25(1): 74-80

Published online January 28, 2015 https://doi.org/10.4014/jmb.1407.07078

Copyright © The Korean Society for Microbiology and Biotechnology.

Effects of Oral Intake of Kimchi-Derived Lactobacillus plantarum K8 Lysates on Skin Moisturizing

Hangeun Kim 1, Hye Rim Kim 2, Bong jun Jeong 2, Seung Su Lee 3, Tae-Rahk Kim 1, 3, Ji Hye Jeong 3, Miyeong Lee 3, Sinai Lee 3, Jong Suk Lee 4 and Dae Kyun Chung 1, 2, 3*

1RNA Inc., College of Life Science, Kyung Hee University, Yongin 449-701, Republic of Korea, 2Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea, 3Skin Biotechnology Center, Gyeonggi Biocenter, Suwon 443-766, Republic of Korea, 4Gyeonggi Institute of Science & Technology Promotion, Suwon 443-270, Republic of Korea

Received: July 28, 2014; Accepted: August 28, 2014

Abstract

Skin is the soft outer covering of vertebrates that provides protection from pathogenic
infection, physical damage, or UV irradiation, and controls body temperature and water
content. In this study, we examined the effects of oral intake of kimchi-derived Lactobacillus
plantarum K8 lysates on skin moisturizing. In an in vitro study, we observed that the
hyaluronic acid content increased in HaCaT cells treated with L. plantarum K8 lysates. Oral
administration of L. plantarum K8 lysates effectively attenuated the horny layer formation and
decreased epidermal thickening in DNCB-treated SKH-1 hairless mice skin. The damage to
barrier function was reduced after 8 weeks of oral administration of L. plantarum K8 lysates as
compared with that in the atopic dermatitis mice. For the test with volunteers, we
manufactured experimental candy containing 2.1% L. plantarum K8 lysates, while control
candy did not contain bacterial lysate. A significant increase in hydration in the experimental
candy-administered group as compared with the control candy-administered group was
observed on the face after 4 and 8 weeks, and on the forearm after 4 weeks. Decreases in horny
layer thickness and TEWL value were observed on the face and forearm of the experimental
group. Together, the in vitro cell line and in vivo mouse studies revealed that L. plantarum K8
lysates have a moisturizing effect. A clinical research study with healthy volunteers also
showed an improvement in barrier repair and function when volunteers took L. plantarum K8
lysates-containing candy. Thus, our results suggest that L. plantarum K8 lysates may help to
improve skin barrier function.

Keywords: Lactobacillus plantarum, L. plantarum K8-LTA, bacterial lysates, atopic dermatitis, aglycone isoflavone, moisturizer

References

  1. Anderson P C, D inulos JG. 2 009. A re t h e new moisturizers more effective? Curr. Opin. Pediatr. 21: 486-490.
    Pubmed CrossRef
  2. Beeckman D, Schoonhoven L, Verhaeghe S, Heyneman A, Defloor T. 2009. Prevention and treatment of incontinenceassociated dermatitis: literature review. J. Adv. Nurs. 65:1141-1154.
    Pubmed CrossRef
  3. Chan LS. 2008. Atopic dermatitis in 2008. Curr. Dir. Autoimmun. 10: 76-118.
    Pubmed CrossRef
  4. Cooper KD. 1994. Atopic dermatitis: recent trends in pathogenesis and therapy. J. Invest. Dermatol. 102: 128-137.
    Pubmed CrossRef
  5. Flynn TC, Petros J, Clark RE, Viehman GE. 2001. Dry skin and moisturizers. Clin. Dermatol. 19: 387-392.
    CrossRef
  6. Georgieva RN, IIiev IN, Chipeva VA, Dimitonova SP, Samelis J, Danova ST. 2008. Identification and in vitro characterization of Lactobacillus plantarum strains from artisanal Bulgarian white brined cheeses. J. Basic Microbiol. 48: 234-244.
    Pubmed CrossRef
  7. Ginsburg I. 2002. Role of lipoteichoic acid in infection and inflammation. Lancet Infect. 2: 171-179.
    CrossRef
  8. Hannuksela A, Kinnunen T. 1992. Moisturizers prevent irritant dermatitis. Acta Derm. Venereol. 72: 42-44.
    Pubmed
  9. Kwon HK, Lee CG, So JS, Chae CS, Hwang JS, Sahoo A, et al. 2010. Generation of regulatory dendritic cells and CD4+Foxp3+ T cells by probiotics administration suppresses immune disorders. Proc. Natl. Acad. Sci. USA 107: 2159-2164.
    Pubmed KoreaMed CrossRef
  10. McCusker MM, Grant-Kels JM. 2010. Healing fats of the skin: the structural and immunologic roles of the omega-6 and omega-3 fatty acids. Clin. Dermatol. 28: 440-451.
    Pubmed CrossRef
  11. Meydani SN, Ha WK. 2000. Immunologic effects of yogurt. Am. J. Clin. Nutr. 71: 861-872.
    Pubmed
  12. Ozdemir O. 2010. Various effects of different probiotic strains in allergic disorders: an update from laboratory and clinical data. Clin. Exp. Immunol. 160: 295-304.
    Pubmed KoreaMed CrossRef
  13. Saijic D, Asiniwasis R, Skotnicki-Grant S. 2012. A look at epidermal barrier function in atopic dermatitis: physiologic lipid replacement and the role of ceramide. Skin Therapy Lett. 7: 6-9.
  14. Seo HS, Michalek SM, Nahm MH. 2008. Lipoteichoic acid is important in innate immune responses to gram-positive bacteria. Infect. Immunol. 76: 206-213.
    Pubmed KoreaMed CrossRef
  15. Simpson E, Böhling A, Bielfeldt S, Bosc C, Kerrouche N. 2013. Improvement of skin barrier function in atopic dermatitis patients with a new moisturizer containing a ceramide precursor. J. Dermatol. Treat. 24: 122-125.
    Pubmed CrossRef
  16. Snapper CM, Paul WE. 1987. Interferon-gamma and B cell stimulatory factor-1 reciprocally regulate Ig isotype production. Science 236: 944-947.
    Pubmed CrossRef
  17. Varothai S, Nitayavardhana S, Kulthanan K. 2013. Moisturizers for patients with atopic dermatitis. Asian Pac. J. Allergy Immunol. 31: 91-98.
    Pubmed
  18. Wallace TD, Bradley S, Buckley ND, Green-Johnson JM. 2003. Interactions of lactic acid bacteria with human intestinal epithelial cells: effects on cytokine production. J. Food Protect. 66: 466-472.
    Pubmed