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Research article

Microbial Ecology and Diversity

Genetic Diversity of Cultivable Plant Growth-Promoting Rhizobacteria in Korea

Won-Il Kim 1*, Won Kyong Cho 2, Su-Nam Kim 3, Hyosub Chu 4, Kyoung-Yul Ryu 1, Jong-Chul Yun 1 and Chang-Seuk Park 5

1Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea, 2Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea, 3Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea, 4Bioindustrial Process Center, Jeonbuk Branch Institute of Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, 580-185, Republic of Korea, 5Department of Applied Biology & Environmental Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea

Received: January 20, 2011; Accepted: May 11, 2011

J. Microbiol. Biotechnol. 2011; 21(8): 777-790

Published August 28, 2011 https://doi.org/10.4014/jmb.1101.01031

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

To elucidate the biodiversity of plant growth-promoting rhizobacteria (PGPR) in Korea, 7,638 bacteria isolated from the rhizosphere of plant species growing in many different regions were screened. A large number of PGPR were identified by testing the ability of each isolate to promote the growth of cucumber seedlings. After redundant rhizobacteria were removed via amplified rDNA restriction analysis, 90 strains were finally selected as PGPR. On the basis of 16S ribosomal RNA sequences, 68 Gram-positive (76%) and 22 Gram-negative (24%) isolates were assigned to 21 genera and 47 species. Of these genera, Bacillus (32 species) made up the largest complement, followed by Paenibacillus (19) and Pseudomonas (11). Phylogenetic analysis showed that most of the Grampositive PGPR fell into two categories: low- and high- G+C (Actinobacteria) strains. The Gram-negative PGPR were distributed in three categories: α-proteobacteria, β- proteobacteria, and γ-proteobacteria. To our knowledge, this is the largest screening study designed to isolate diverse PGPR. The enlarged understanding of PGPR genetic diversity provided herein will expand the knowledge base regarding beneficial plant-microbe interactions. The outcome of this research may have a practical effect on crop production methodologies.

Keywords

PGPR, plant growth promotion, diversity, rhizobacteria, cucumber, rhizosphere

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Research article

J. Microbiol. Biotechnol. 2011; 21(8): 777-790

Published online August 28, 2011 https://doi.org/10.4014/jmb.1101.01031

Copyright © The Korean Society for Microbiology and Biotechnology.

Genetic Diversity of Cultivable Plant Growth-Promoting Rhizobacteria in Korea

Won-Il Kim 1*, Won Kyong Cho 2, Su-Nam Kim 3, Hyosub Chu 4, Kyoung-Yul Ryu 1, Jong-Chul Yun 1 and Chang-Seuk Park 5

1Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea, 2Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea, 3Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration (RDA), Suwon, 441-707, Republic of Korea, 4Bioindustrial Process Center, Jeonbuk Branch Institute of Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeonbuk, 580-185, Republic of Korea, 5Department of Applied Biology & Environmental Sciences, Gyeongsang National University, Jinju 660-701, Republic of Korea

Received: January 20, 2011; Accepted: May 11, 2011

Abstract

To elucidate the biodiversity of plant growth-promoting
rhizobacteria (PGPR) in Korea, 7,638 bacteria isolated
from the rhizosphere of plant species growing in many
different regions were screened. A large number of PGPR
were identified by testing the ability of each isolate
to promote the growth of cucumber seedlings. After
redundant rhizobacteria were removed via amplified
rDNA restriction analysis, 90 strains were finally selected
as PGPR. On the basis of 16S ribosomal RNA sequences,
68 Gram-positive (76%) and 22 Gram-negative (24%)
isolates were assigned to 21 genera and 47 species. Of
these genera, Bacillus (32 species) made up the largest
complement, followed by Paenibacillus (19) and Pseudomonas
(11). Phylogenetic analysis showed that most of the Grampositive
PGPR fell into two categories: low- and high-
G+C (Actinobacteria) strains. The Gram-negative PGPR
were distributed in three categories: α-proteobacteria, β-
proteobacteria, and γ-proteobacteria. To our knowledge,
this is the largest screening study designed to isolate
diverse PGPR. The enlarged understanding of PGPR genetic
diversity provided herein will expand the knowledge base
regarding beneficial plant-microbe interactions. The
outcome of this research may have a practical effect on
crop production methodologies.

Keywords: PGPR, plant growth promotion, diversity, rhizobacteria, cucumber, rhizosphere