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Research article

Biomolecular and Metabolic Engineering

Production of Biosurfactant Lipopeptides Iturin A, Fengycin and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides

Pyoung Il Kim 1, Jaewon Ryu 2, Young Hwan Kim 3 and Youn-Tae Chi 4*

1School of Chemical and Biological Engineering Institute of Molecular Biology and Genetics, Seoul National University, 2Department of Biotechnology, Graduate School, Chonnam National University, 3Proteomics Team, Korea Basic Science Institute, 4School of Biological Sciences and Technology and Biotechnology Research Institute, Chonnam National University

Received: May 8, 2009; Accepted: August 14, 2009

J. Microbiol. Biotechnol. 2010; 20(1): 138-145

Published January 28, 2010 https://doi.org/10.4014/jmb.0905.05007

Copyright © The Korean Society for Microbiology and Biotechnology.

Abstract

A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30˚C. Biosurfactant lipopeptides produced by Bacillus subtilis CMB32 were precipitated by adjusting to pH 2 and extracting using chloroform/methanol, and then were purified using column chromatography and reverse-phase HPLC. Molecular masses of the lipopeptides were estimated by MALDI-TOF mass spectrometry as (a) 1080, (b) 1486, and (c) 1044 Da, respectively. They had cyclic structures and amino acid compositions of (a) Pro, Asx, Ser, Tyr, Glx, (b) Glx, Tyr, Thr, Ala, Pro, Ile, and (c) Glx, Leu, Val, Asx, respectively. Further analysis revealed that Bacillus subtilis CMB32 produced three antifungal lipopeptides: (a) iturin A, (b) fengycin, and (c) surfactin A.

Keywords

Bacillus subtilis CMB32, Colletotrichum gloeosporioides, Iturin A, Fengycin, Surfactin A, MALDI-TOF mass spectrometry

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Research article

J. Microbiol. Biotechnol. 2010; 20(1): 138-145

Published online January 28, 2010 https://doi.org/10.4014/jmb.0905.05007

Copyright © The Korean Society for Microbiology and Biotechnology.

Production of Biosurfactant Lipopeptides Iturin A, Fengycin and Surfactin A from Bacillus subtilis CMB32 for Control of Colletotrichum gloeosporioides

Pyoung Il Kim 1, Jaewon Ryu 2, Young Hwan Kim 3 and Youn-Tae Chi 4*

1School of Chemical and Biological Engineering Institute of Molecular Biology and Genetics, Seoul National University, 2Department of Biotechnology, Graduate School, Chonnam National University, 3Proteomics Team, Korea Basic Science Institute, 4School of Biological Sciences and Technology and Biotechnology Research Institute, Chonnam National University

Received: May 8, 2009; Accepted: August 14, 2009

Abstract

A bacterial strain isolated from soil for its potential to control the anthracnose disease caused by Colletotrichum gloeosporioides was identified as a Bacillus subtilis. Bacillus subtilis CMB32 produced antifungal agents on M9 broth at 30˚C. Biosurfactant lipopeptides produced by Bacillus subtilis CMB32 were precipitated by adjusting to pH 2 and extracting using chloroform/methanol, and then were purified using column chromatography and reverse-phase HPLC. Molecular masses of the lipopeptides were estimated by MALDI-TOF mass spectrometry as (a) 1080, (b) 1486, and (c) 1044 Da, respectively. They had cyclic structures and amino acid compositions of (a) Pro, Asx, Ser, Tyr, Glx, (b) Glx, Tyr, Thr, Ala, Pro, Ile, and (c) Glx, Leu, Val, Asx, respectively. Further analysis revealed that Bacillus subtilis CMB32 produced three antifungal lipopeptides: (a) iturin A, (b) fengycin, and (c) surfactin A.

Keywords: Bacillus subtilis CMB32, Colletotrichum gloeosporioides, Iturin A, Fengycin, Surfactin A, MALDI-TOF mass spectrometry