Fig. 3. β-Carotene production and cell growth in test tube culture.
(A) β-carotene roduction in pSTV28-pβCA (no MVA expression), pSNA-pβCA (BSL2 MVA construct), pSCS1- pβCA, pSCS2- pβCA, and pSCS3-pβCA (BSL1 MVA constructs) after 24 h and 48 h cultivation (B) Cell growth measurement at OD600. (C) Cell-specific productivity of β-carotene. Data are shown as average ± SD of three biological replicates. Asterisks in panels A and C indicate statistical significance as follows: * (p ≤ 0.05), and ** (p ≤ 0.01), compared to the pSNA-pβCA strain.