Plasmids |
Description |
Ref. |
pSTV28 |
Plac, pACYC184 ori, lacZ, Cmr |
Takara Co., Ltd. |
pTrc99A |
Ptrc, pBR322 ori, lacIq, Ampr |
Amersham Biosci. |
pSNA |
pSTV28 containing mvaE and mvaS of E. faecalis, mvaK1, mvaK2, and mvaD of S. pneumoniae, and idi of E. coli |
[2] |
pT-LYCm4 |
pTrc99A with crtE, crtB, and crtI of Pantoea agglomerans, and ipiHP1 of H. pluvialis |
[6] |
pT-HB |
pT-LYCm4 with crtY of Pantoea ananatis |
[7] |
pSCS1 |
pSTV28 containing E. coli codon-optimized EsmvaE and EsmvaS from E. saccharolyticus, E. coli codon-optimized erg12, erg19, and erg8 from S. cerevisiae and idi from E. coli |
This study |
pSCS2 |
pSTV28 containing E. coli codon-optimized EsmvaE and EsmvaS from E. saccharolyticus, E. coli codon-optimized erg12, erg19, and erg8 from S. cerevisiae and E. coli codonoptimized idi from E. coli |
This study |
pSCS3 |
pSTV28 containing E. coli codon-optimized EsmvaE and EsmvaS from E. saccharolyticus, E. coli codon-optimized erg12, erg19, and erg8 from S. cerevisiae and E. coli codonoptimized fni from B. subtilis |
This study |
Strains |
Description |
Ref. |
DH5α |
F−, ϕ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1 endA1, hsdR17(rK− mK+), phoA, supE44, λ−, thi-1, gyrA96, relA1 |
Lab collection |
pSTV28-pLYC |
E. coli harboring pSTV28 and pT-LYCm4 |
This study |
pSNA-pLYC |
E. coli harboring pSNA and pT-LYCm4 |
This study |
pSCS1-pLYC |
E. coli harboring pSCS1 and pT-LYCm4 |
This study |
pSCS2-pLYC |
E. coli harboring pSCS2 and pT-LYCm4 |
This study |
pSCS3-pLYC |
E. coli harboring pSCS3 and pT-LYCm4 |
This study |
pSTV28-pβCA |
E. coli harboring pSTV28 and pT-HB |
This study |
pSNA-pβCA |
E. coli harboring pSNA and pT-HB |
This study |
pSCS1-pβCA |
E. coli harboring pSCS1and pT-HB |
This study |
pSCS2-pβCA |
E. coli harboring pSCS2 and pT-HB |
This study |
pSCS3-pβCA |
E. coli harboring pSCS3 and pT-HB |
This study |