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Table. 1.

Table. 1.

Plasmids and strains used in this study.

Plasmids Description Ref.
pSTV28 Plac, pACYC184 ori, lacZ, Cmr Takara Co., Ltd.
pTrc99A Ptrc, pBR322 ori, lacIq, Ampr Amersham Biosci.
pSNA pSTV28 containing mvaE and mvaS of E. faecalis, mvaK1, mvaK2, and mvaD of S. pneumoniae, and idi of E. coli [2]
pT-LYCm4 pTrc99A with crtE, crtB, and crtI of Pantoea agglomerans, and ipiHP1 of H. pluvialis [6]
pT-HB pT-LYCm4 with crtY of Pantoea ananatis [7]
pSCS1 pSTV28 containing E. coli codon-optimized EsmvaE and EsmvaS from E. saccharolyticus, E. coli codon-optimized erg12, erg19, and erg8 from S. cerevisiae and idi from E. coli This study
pSCS2 pSTV28 containing E. coli codon-optimized EsmvaE and EsmvaS from E. saccharolyticus, E. coli codon-optimized erg12, erg19, and erg8 from S. cerevisiae and E. coli codonoptimized idi from E. coli This study
pSCS3 pSTV28 containing E. coli codon-optimized EsmvaE and EsmvaS from E. saccharolyticus, E. coli codon-optimized erg12, erg19, and erg8 from S. cerevisiae and E. coli codonoptimized fni from B. subtilis This study
Strains Description Ref.
DH5α F−, ϕ80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1 endA1, hsdR17(rK− mK+), phoA, supE44, λ−, thi-1, gyrA96, relA1 Lab collection
pSTV28-pLYC E. coli harboring pSTV28 and pT-LYCm4 This study
pSNA-pLYC E. coli harboring pSNA and pT-LYCm4 This study
pSCS1-pLYC E. coli harboring pSCS1 and pT-LYCm4 This study
pSCS2-pLYC E. coli harboring pSCS2 and pT-LYCm4 This study
pSCS3-pLYC E. coli harboring pSCS3 and pT-LYCm4 This study
pSTV28-pβCA E. coli harboring pSTV28 and pT-HB This study
pSNA-pβCA E. coli harboring pSNA and pT-HB This study
pSCS1-pβCA E. coli harboring pSCS1and pT-HB This study
pSCS2-pβCA E. coli harboring pSCS2 and pT-HB This study
pSCS3-pβCA E. coli harboring pSCS3 and pT-HB This study
J. Microbiol. Biotechnol. 2024;34:2338~2346 https://doi.org/10.4014/jmb.2408.08053
© J. Microbiol. Biotechnol.