eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 1.
Table. 1.
List and sequences of primers used in plasmid construction and qRT-PCR.
| Gene | Sequence (5'–3') | Tm (°C) | Description | |
|---|---|---|---|---|
| GLP | F | GCTGTGACTGCTGGTACTGT | 61.0 | GLP |
| R | CGCGGACGAGAGAATAGCAA | 61.3 | ||
| SP1 | F | TCTACTTCCTGCCCGACTACAAC | 63.1 | Starch Phosphorylase |
| R | GAACACGAACACCTCCTCCAC | 63.9 | ||
| SP2 | F | TCTACTTCCTGCCCGACTACAAC | 63.4 | Starch Phosphorylase |
| R | GAACACGAACACCTCCTCCAC | 58.2 | ||
| GPDH | F | AACACGCTGCACGAAAACAC | 60.0 | Glycerol 3-phosphate dehydrogenase |
| R | TTGCTGACGCAGATGATGG | 59.7 | ||
| PFOR | F | CGTGGCGGTGTTTGAGA | 60.9 | Pyruvate–ferredoxin oxidoreductase |
| R | GGTGTTGCTGGCGATGA | 60.6 | ||
| FDX | F | CGGGCAAGACGAAGACTATGG | 63.1 | Ferredoxin |
| R | GGTAGGCGGAGCACATGAG | 63.4 | ||
| HSF1 | F | AACATCGTCTCATGGGGTGC | 62.7 | Heat shock factor 1 |
| R | TCCATAGGTGTTGAGCTGGC | 62.1 | ||
| CAT2 | F | TCAGCAACCAGTACTTCAAGGTG | 61.5 | Catalase |
| R | ATCTCAGCGTCCCACTTGATCG | 63.9 | ||
| APX2 | F | AGCCCTGGAACAACACAAAGGAC | 65.0 | Ascorbate peroxidase |
| R | AGAAGTCGCGGAAGAACAGATCC | 63.7 | ||
| AOX1 | F | AGAGGTGATTCGTGTGCCTG | 62.2 | Alternative oxidase |
| R | CCAGGCACACAGATCCAAGT | 62.7 | ||
| Cr_Actin | F | TGTGCATACGTGGATAGCTTG | 58.2 | Actin |
| R | ATGACCCGCTCCTCATATCTT | 59.9 | ||
| Tub A | F | CTCGCTTCGCTTTGACGGTG | 62.5 | Tubulin |
| R | CGGCTCTTCCGCATGGTGC | 63.8 | ||
J. Microbiol. Biotechnol. 2024;34:2132~2141 https://doi.org/10.4014/jmb.2407.07059
© J. Microbiol. Biotechnol.