Fig. 7.

Fig. 7. Effect of HPH on dorsal skin barrier in DNCB-sensitized BALB/c mice. After autopsy, dorsal skin tissue samples were obtained from individual mice from each group. (A) The typical histological features of the dorsal skin tissues of BALB/c mice in which AD had been induced were visualized using H&E staining. (B and C) The dorsal skin tissue sections were stained with anti- filaggrin and TSLP antibodies on BALB/c mice in which AD had been induced. (D) The epidermal thickness of individual mice from each group was quantified. (E and F) The filaggrin and TSLP graphs display mean values. In a microscopic image of the dorsal skin tissue, three arbitrary points (left, center, and right) were selected from the entire length of the skin tissue, and images of the points were taken at ×200 magnification. In each of the latter images, two points were arbitrarily selected. In a total of six points/mouse, thickness was measured from the stratum corneum to the basement membrane using i-Solution Lite. Data are expressed as the mean ± SD. **p < 0.01, ***p < 0.001 compared with the control group. ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 compared with the normal group. N, normal group; C, DNCB-induced control group; HPH200, HPH 200 μl-administered group; HPH400, HPH 400 μl-administered group; DEX, dexamethasone 10 mg/kg-administered group. Scale bar = 50 μm. e, epidermis; d, dermis. Black arrows indicate mast cells.

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