Fig. 2. Nematicidal activities of isolated bacteria against the J2s of four Meloidogyne species.
(A) Mortality rate of the J2s of Meloidogyne after 48 h treated with 10% cell-free filtrates of isolated bacteria. Sunchungtan 150EC (150 μg/ml of fosthiazate) were used as the positive control, while LB broth was used as the negative control. (B) Morphological observation of the assessed nematodes with active nematodes (upper) and the dead nematodes with straight form and immobility (below) post-stimulation using a fine needle. All experiments were performed in triplicate wells and repeated three times under the same conditions. Different letters above the error bars indicate significant differences by Scheffé's test (p < 0.05). Scale bar: 20 μm.