Fig. 3.

Fig. 3. BI sup attenuates the cohesion of 3D spheroid cancer cells. (A and B) 3D spheroid formation assay with the HCT116, SNU-C5, MB-231 and HS578T cell lines. Cells treated with BI sup were loaded onto ULA plates and incubated for 2 days. The cells were photographed under a microscope each day. Scale bar, 500 μm (left). The size of the spheroids was measured using ImageJ software (right panel). The mean ± SD of three independent experiments are shown. All p values were calculated using Student’s t tests (***p < 0.001). (C) qRT‒PCR analysis of EMT-related genes in 3D spheroid cancer cells after treatment with BI sup. All p values were calculated using Student’s t test (***p < 0.001, **p < 0.01, *p < 0.05).

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