Fig. 1.

Fig. 1. BI sup induces cell death in colorectal and breast cancer cell lines. (A) Cell growth assay after treatment with MRS and BI sup for 48 h. HCT116, SNU-C5, MB-231 and Hs578t cells were fixed in 100% methanol and stained with crystal violet solution. Scale bar, 500 μm. (B) CCK-8 assay. After the addition of CCK-8 solution, the cells were incubated for 5 min at 37°C. The intensity of cell growth was measured using a microplate reader (450 nm). The mean ± SD of three independent experiments are shown. All p values were calculated using Student’s t test (***p < 0.001). (C) DAVID-based GO analysis of the RNA-seq results for the 2908 genes upregulated by BI sup. The enriched terms are shown. (D) Heatmap analysis of RNA-seq results for EMT-related genes. The log2 p value of the BI sup divided by the MRS ratio was analyzed, and gene selection was conducted using a threshold log2 p value of +1 or greater or -1 or less.

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