eISSN 1738-8872
pISSN 1017-7825
pISSN 1017-7825
Table. 2.
Table. 2.
Specific activity (37°C), kinetic parameters (37°C), thermodynamic parameters, and protease resistance of the recombinant phytase.
| Recombinant phytase | Specific activity (U/mg) | 55°C |
80°C |
Pepsin resistance (%) | Trypsin resistance (%) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| YiAPPA | 3960.81 ± 105.34 | 126.24 ± 14.18 | 10375.27 ± 1256.73 | 82.19 | 30.09 ± 0.19 | 14.81 ± 0.37 | 55.12 ± 0.87 | 48.36 ± 0.53 | 84.17 ± 3.20 | 82.28 ± 4.18 |
| K189R/K216R | 4469.13 ± 154.19 | 111.59 ± 11.03 | 10363.29 ± 987.56 | 92.87 | 60.45 ± 0.63 | 23.35 ± 0.57 | 62.44 ± 0.76 | 53.18 ± 0.62 | 85.43 ± 4.09 | 83.97 ± 3.57 |
| K189R | 3979.56 ± 121.00 | 125.30 ± 13.09 | 10301.32 ± 1107.83 | 82.21 | 47.34 ± 0.38 | 17.86 ± 0.51 | 57.32 ± 0.64 | 49.93 ± 0.32 | 84.36 ± 2.98 | 83.01 ± 5.03 |
| K216R | 4352.96 ± 121.15 | 115.01 ± 9.86 | 10381.09 ± 1091.32 | 90.26 | 50.09 ± 0.52 | 18.90 ± 0.43 | 59.85 ± 0.73 | 50.79 ± 0.57 | 84.91 ± 3.25 | 83.30 ± 4.18 |
J. Microbiol. Biotechnol. 2024;34:1660~1670 https://doi.org/10.4014/jmb.2403.03031
© J. Microbiol. Biotechnol.