Fig. 1.

Fig. 1. Effect of HDB7040 on MCF-7 cell viability and proliferation under EF conditions. (A) MCF-7 cells were treated with various concentrations of HDB7040 (0-1,000 μg/ml) for 24 h, and cell viability was investigated by MTT assay. (B) HDB7040-induced MCF-7 cell proliferation upon EF condition was assessed by E-screen assay, and (C) cellular morphology was monitored using a light microscope. (D) The effect of HDB7040 on ER activation was confirmed by an E-screen assay using an ER antagonist. 17β-estradiol (E2) was used as a positive control. Data are presented as the mean ± SEM (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001, compared to the control; ##, p < 0.01, compared to the non-ICI-treated group.

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