Characteristics of representative collagenolytic and thermostable collagenolytic proteases from diverse microorganisms.
Collagenolytic proteases | Optimal temperature (°C) and pH | Size (kDa) | Family |
---|---|---|---|
TSS from |
70°C; pH 9.0 | 58.0 | S8 |
MO-1 from |
60°C; pH 7.1-9.3 | 105.0 | S8 |
MCP-01 from |
60°C; pH 9.0 | 65.8 [66] | S8 |
AcpII from |
45°C; pH 8.5-9.0 | 55.0 | S8 |
21E from |
70-75°C; pH 9.0-9.5 | 50.0 | S8 |
Myroicolsin from |
60°C; pH 8.5 | 56.0 | S8 |
SPSFQ from |
40°C; pH 9.0 | 30.0 | S8 |
SOT from |
55°C; pH 8.0-9.0 | 24.0 | S1 |
SGT from |
55°C; 7.5-8.6 | 24.0 | S1 |
Kumamolisin-As from |
60°C; pH 4.0 | 37.0 | S53 |
Kumamolisin-Ac from |
60°C; pH 2.0 | 45.0 | S53 |
ColG/ColH from |
37°C; pH 7.4 (6.3-8.0) [74] | 116.0 | M9B |
ColT from |
37°C; pH 7.4 (6.3-8.0) [74] | 114.0 [31] | M9B |
ColA from |
42°C; pH 7.0-7.2 | 116.0 | M9B |
VMC from |
30-40°C; pH 8.0 [88] | 63.0 | M9A |
VHC from |
30-40°C; pH 7.0-8.0 | 62.0 | M9A |
MPK from |
60°C; pH 7.5 | 61.0 | Cysteine protease |