Fig. 2.

Fig. 2. Feasibility of the proposed method for P. aeruginosa detection. (A) Fluorescence spectrum of Cy5 labeled aptamer before and after being assembled into capture probe. (B) Fluorescence spectrum of Cy5 labeled capture probe before and after being fixed on the surface of MNPs. (C) SYBR Green I signals of the EXPAR process with different incubation time. (D) Cy5 signals of the L2 probe during the CHA process. column 1: L2 probe in linear state, column 2: L2 probe, column 3: L2+ L1, column 4: L2+ “4”, column 5: L2+ L1+ “4”. (E) ThT signals of the method when essential components existed or not. column 1: target (-), column 2: p-EXPAR (-), column 3: CHA (-), column 4: ThT (-), column 5: with all. Data were expressed as mean ± standard deviations, n = 3 technical replicates.

© J. Microbiol. Biotechnol.