Fig. 2. Feasibility analysis of the proposed method.
(A) Fluorescence spectrum of the FAM labeled recognizing probe when MRSA exists or not. (B) Fluorescence intensities of the recognizing probe for target and interfering molecules detection. (C) Fluorescence intensities of the recognizing probe under different experimental conditions. (D) Fluorescence spectrum of the FAM labeled split DNAzyme before and after proximity ligation of split DNAzyme. (E) Fluorescence intensities of the FAM labeled LR probe during the catalytic hairpin assembly process. (F) Absorbance of the approach during MRSA detection. Inserted is the visible absorbance changes. n = 3 technical duplicates.