Fig. 6.

Fig. 6. FUT1 overexpression reverses the inhibitory effect of LINC00511 knockdown on LPS-induced chondrocyte pyroptosis. LINC00511 was silenced while FUT1 was overexpressed in LPS-treated ATDC5 cells. A and B, FUT1 expression measured by qRT‒PCR (A) and western blot analysis (B). C and D, Levels of IL-1β and IL-18 verified by qRT‒PCR (C) and ELISA (D). E and F, The levels of NLRP3, ASC, Caspase-1, and GSDMD were detected via qRT‒PCR (E) and western blot analysis (F). (G) Cell viability was analysed by Cell Titer Glo Luminescent Cell Viability Assay kits. (H) LDH viability measurement. Three independent repeated tests were conducted. The results are presented as the mean ± standard deviation. One-way ANOVA was used to analyse the data in Panels A, B, G, and H. Two-way ANOVA was used to analyse the data in Panels C, D, E, and F. Tukey's multiple comparisons test was used for post hoc analysis. *p < 0.05.

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