Fig. 5. LINC00511 knockdown sponges miR-9-5p to quench FUT1 expression in LPS-induced chondrocyte pyroptosis.
(A) The binding relationship between miR-9-5p and FUT1 was predicted by the starBase website (http:// starbase.sysu.edu.cn/index.php). (B) and (C) The binding relationship between miR-9-5p and FUT1 was verified by dualluciferase reporter gene assays (B) and RIP assays (C). (D) and (F) Levels of FUT1 or miR-9-5p determined by qRT‒PCR. E and G, Levels of FUT1 determined by Western blot analysis (E, G). Three independent repeated tests were conducted. The results are presented as the mean ± standard deviation. One-way ANOVA was used to analyse the data in Panels D and E. Two-way ANOVA was used to analyse the data in Panels B and C. Tukey's multiple comparisons test was applied for the post hoc test. *p < 0.05.