Fig. 4.

Fig. 4. miR-9-5p underexpression neutralizes the inhibitory effect of LINC00511 knockdown on LPSinduced chondrocyte pyroptosis. LINC00511 and miR-9-5p were both silenced in LPS-treated ATDC5 cells. (A) miR-9-5p expression was examined via qRT‒PCR. B and C, Levels of IL-1β and IL-18 determined by qRT‒PCR (B) and ELISA (C). D and E, The levels of NLRP3, ASC, Caspase-1, and GSDMD were detected via qRT‒PCR (D) and western blot analysis (E). (F) Cell viability was tested with Cell Titer Glo Luminescent Cell Viability Assay kits. (G) LDH viability measurement. Three independent repeated tests were conducted. The results are presented as the mean ± standard deviation. One-way ANOVA was used to analyse the data in Panels A, F, and G. Two-way ANOVA was used to analyse the data in Panels B, C, D, and E. Tukey's multiple comparisons test was used for post hoc analysis. *p < 0.05.

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