Fig. 2.

Fig. 2. LINC00511 knockdown inhibits the NLRP3 inflammasome to limit LPS-induced chondrocyte pyroptosis. si-NC and si-LINC00511 were transfected into LPS-treated ATDC5 cells. (A) LINC00511 expression was examined via qRT‒PCR. B and C, Levels of IL-1β and IL-18 determined by qRT‒PCR (B) and ELISA (C). D and E, The levels of NLRP3, ASC, Caspase-1, and GSDMD were detected via qRT‒PCR (D) and western blot analysis (E). (F) Caspase-1 levels were assessed by dual-luciferase reporter gene assays at 400×; scale bar = 25 μm. (G) Cell viability was tested with Cell Titer Glo Luminescent Cell Viability Assay kits. (H) LDH viability measurement. Three independent repeated tests were conducted. The results are presented as the mean ± standard deviation. Independent t tests were used to analyse the data in Panels A, F, G, and H. Two-way ANOVA was used to analyse the data in Panels B, C, D, and E. Tukey's multiple comparisons test was used for post hoc analysis. *p < 0.05.

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