Primer sequences for qRT‒PCR.
Forward Primer (5'-3') | Reverse Primer (5'-3') | |
---|---|---|
LINC00511 | CGGCTTAACTAACTGTTACTC | CAGTACCGATGTCAGACACGGA |
miR-9-5p | GGAGTCCGTGTGTCTGTGTG | GCTTTATGA CGGCTCTGTGG |
FUT1 | AAAGCGGACTGTGGATCT | GGACACAGGATCGACAGG |
IL-1β | CTTCAGGCAGGCAGTATCACTC | TGCAGTTGTCTAATGGGAACGT |
IL-18 | GCCTCAAACCTTCCAAATCA | TGGATCCATTTCCTCAAAGG |
NLRP3 | AGCCTTCCAGGATCCTCTTC | CTTGGGCAGCAGTTTCTTTC |
ASC | GACAGTGCAACTGCGAGAAG | CGACTCCAGATAGTAGCTGACAA |
Caspase-1 | ACACGTCTTGCCCTCATTATCT | ATAACCTTGGGCTTGTCTTTCA |
GSDMD | CCAACATCTCAGGGCCCCAT | TGGCAAGTTTCTGCCCTGGA |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |
GAPDH | CAGTCACTACTCAGCTGCCA | GAGGGTGCTCC GGTAG |
qRT‒PCR, quantitative real-time polymerase chain reaction; LINC, long intergenic nonprotein coding RNA; miR, microRNA; FUT1, fucosyltransferase 1; IL, interleukin; NLRP3, nod-like receptor pyrin domain 3; ASC, apoptosis-associated speck-like protein; GSDMD, gasdermin D, GAPDH, glyceraldehyde-3-phosphate dehydrogenase