Fig. 1.

Fig. 1. LPS activates the NLRP3 inflammasome to induce caspase-1-induced chondrocyte pyroptosis and upregulates LINC00511 expression. ATDC5 cells were treated with LPS to induce chondrocyte inflammatory injury. Cells without LPS treatment were used as the control group. A and B, Levels of IL-1β and IL-18 verified by qRT‒PCR (A) and ELISA (B). (C) and (D), The levels of NLRP3, ASC, Caspase-1, and GSDMD were tested using qRT‒PCR (C) and western blot analysis (D). (E) Caspase-1 levels were assessed by dual-luciferase reporter gene assays at 400×; scale bar = 25 μm. (F) Cell viability was analysed by Cell Titer Glo Luminescent Cell Viability Assay kits. (G) LDH viability measurement. (H) LINC00511 expression was tested through qRT‒PCR. Three repeated tests were performed. The results are presented as the mean ± standard deviation. Two-way ANOVA was used to analyse the data in Panels A, B, C, and D. Tukey's multiple comparisons test was used for the post hoc test. Independent t tests were used to analyse the data in Panels E, F, G, and H. *p < 0.05.

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