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Table. 2.

Table. 2.

Kinetic parameters for TmPhy and mutants.

Km (mM) kcat (s-1) kcat/Km (s-1mM-1)
TmPhy 0.56 ± 0.05 112.4 ± 11.1 200.5 ± 19.8
TmPhyM 0.30 ± 0.03 115.9 ± 6.5 391.4 ± 22.1
TmPhyMD1 0.32 ± 0.05 3.8 ± 0.4 12.0 ± 1.2
TmPhyMD2 0.28 ± 0.02 205.6 ± 9.0 742.9 ± 32.7
TmPhyMD3 0.36 ± 0.02 80.0 ± 3.2 221.7 ± 8.9

All parameters were measured at pH5.5 and 37°C.

J. Microbiol. Biotechnol. 2024;34:1119~1125 https://doi.org/10.4014/jmb.2311.11044
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