Fig. 4. YBS1 inhibits the early differentiation of 3T3-L1 pre-adipocytes through p38 MAPK signaling.
(A and B) Expression and quantification of p38 MAPK phosphorylation levels between cells treated with and without 5 μl/ml YBS1 for 0.5, 1, 3, 6, and 12 h. (C and D) Phosphorylation levels and quantification of p38 MAPK in 3T3-L1 pre-adipocytes treated with YBS1 in combination with or without SB203580 for 1 h. Protein expression level was quantified using the Image J program. (E and F) mRNA expression level after treatment with SB203580; ns, *, **, and *** indicate non-significance, p < 0.05, p < 0.01, and p < 0.001, respectively. MDI: 0.5 mM isobutylmethylxanthine, 1 μM dexamethasone, and 10 μg/ml insulin; MRS, de Man, Rogosa, and Sharpe medium (5 μl/ml).