Fig. 5.

Fig. 5. Quality of the purified scFv Herceptin and Maa48 Fab expressed under different conditions. SDS-PAGE of scFv Herceptin (A) and Maa48 Fab (B) expressed in either BL21(DE3)+CyDisCo (1), SHuffle T7 Express (2), MG1655+CyDisCo (3) or SHuffle T7 (4). Samples are analyzed as untreated under reducing conditions and as pretreated either with N-Ethylmaleimide or malPEG5000 under non-reducing conditions (NR+NEM or NR+malPEG, respectively). Box (solid line) surround the position of the POI under the different conditions, dotted box point a co-eluted contaminant. In this image, contrast and brightness have been modified to get better visualization of the bands. To see the original image, see Fig. S6.

© J. Microbiol. Biotechnol.