Fig. 3.

Fig. 3. Protein production from B strains from a Ptac promoter in chemically defined media. (A) IMACpurified yields of POI. Bars are mean + SD (n = 3). p ≤ 0.01 (**); p ≤ 0.001 (***) (B) Reduced SDS-PAGE analysis of soluble cell lysates for POI produced using SHuffle (S) or CyDisCo (C). 1 ScFv Herceptin (27.2 kDa); 2) scFv 3211 (27.9 kDa); 3) Herceptin Fab (25.3 kDa, 23.6 kDa); 4) MAA48 Fab (24.7 kDa, 23.3 kDa); 5) B4GalT1 ((35.1 kDa). The strains used were SHuffle T7 Express and BL21(DE3)+CyDisCo.

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