JMB

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Table. 1.

Table. 1.

Proteins of interest (POI) used in this study with their respective sizes and number of disulfide bonds.

POI Size (kDa) Disulfide bonds Ref.
Human growth hormone (hGH) 23.2 2 [17, 31]
E. coli alkaline phosphatase (PhoA) 47.5 2 [11, 14]
Humanized scFv Herceptin 27.2 2 [16, 32]
Human scFv 3M80 27.3 2 [16, 17]
Mouse scFv 3211 27.9 2 [16]
Humanized Herceptin Fab 48.8 5 (i) [16, 18]
Human Maa48 Fab 47.9 5 (i) [16, 18]
Human Beta-1,4-galactosyltransferase 1 (B4Galt1) 35.1 2 [33]
Human Angiopoietin-2 (Ang-2) fibrinogen domain 16.6 3 unpublished
Chicken Avidin 16.7 1 [17]
Gaussia princeps luciferase (GLuc) 19.2 5 [11, 34]
E. coli phytase (AppA) 42.4 4 [11, 14]
Bovine pancreatic trypsin inhibitor (BPTI) 7.60 3 [15]
Human interferon alpha 2b (IFNα-2b) 20.7 2 [15, 31]

Size marked includes an N-terminal hexa-histidine purification tag. (i) indicates that it includes an intramolecular disulfide bond. References refer to previously reported production, in the cytoplasm of E. coli.

J. Microbiol. Biotechnol. 2024;34:1126~1134 https://doi.org/10.4014/jmb.2311.11025
© J. Microbiol. Biotechnol.