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Fig. 4.

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Fig. 4. Celery (A. graveolens) Scaffold Preparation and Cell Seeding. (A) This visual representation depicts the process of celery scaffold preparation, crucial for subsequent tissue engineering applications. (B) The samples obtained were 6mm wide with a thickness of 2.15 ± 0.15 mm. "XY" denotes scaffolds cut longitudinally (left) relative to the celery stalk, while "CS" corresponds to cross sections (right). After a 3-day incubation period in 0.1% SDS, the samples became clear, indicating successful decellularization. (C) Decellularized scaffolds served as substrates for cell seeding, with approximately 50,000 cells applied and left on the scaffold for 4.5 h. This crucial step initiates cell attachment and colonization onto the scaffold matrix. The presence of the vascular bundle (V.B.) within the scaffold underscores its anatomical relevance and potential for vascularized tissue engineering approaches. This method offers a promising platform for developing tissue-engineered constructs using natural plant-based scaffolds. The transparency achieved post-decellularization facilitates cell visualization and analysis, aiding in the evaluation of cell behavior and tissue formation. This comprehensive approach bridges the gap between plant biology and tissue engineering, offering novel strategies for scaffold fabrication and cell seeding techniques. Further exploration of celery-derived scaffolds holds significant promise for regenerative medicine applications and organ-ona- chip technologies. (Reproduced, with permission, copyright 2020, bioRxiv)
J. Microbiol. Biotechnol. 2024;34:1003~1016 https://doi.org/10.4014/jmb.2401.01024
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