eISSN 1738-8872
pISSN 1017-7825
Fig. 1.
Download original image
Fig. 1.
Sensitivity comparison between the SSU, SWP, and ITS-1 PCR assays determined by a 10-fold dilution (from 10
−1
to 10
−5
) of EHP-positive DNA (21-079B1).
Lane M: 100 bp DNA ladder; Lane N: negative control (DEPCwater). (
A
) SWP-PCR first-step (514 bp) and nested-step (148 bp) PCR result. Lanes 1-5: 10
−1
to 10
−5
dilution (first step); Lanes 6-10: 10
−1
to 10
−5
dilution (nested step). (
B
) SSU-PCR (510 bp) result. Lanes 1-5: 10
−1
to 10
−5
dilution. (
C
) ITS-1-PCR first-step (603 bp) and nested-step (400 bp) PCR result. Lanes 1-5: 10
−1
to 10
−5
dilution (first step); Lanes 6-10: 10
−1
to 10
−5
dilution (nested step).
J. Microbiol. Biotechnol. 2024;34:1146~1153
https://doi.org/10.4014/jmb.2401.01010
© J. Microbiol. Biotechnol.