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Fig. 1.

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Fig. 1. Sensitivity comparison between the SSU, SWP, and ITS-1 PCR assays determined by a 10-fold dilution (from 10−1 to 10−5) of EHP-positive DNA (21-079B1). Lane M: 100 bp DNA ladder; Lane N: negative control (DEPCwater). (A) SWP-PCR first-step (514 bp) and nested-step (148 bp) PCR result. Lanes 1-5: 10−1 to 10−5 dilution (first step); Lanes 6-10: 10−1 to 10−5 dilution (nested step). (B) SSU-PCR (510 bp) result. Lanes 1-5: 10−1 to 10−5 dilution. (C) ITS-1-PCR first-step (603 bp) and nested-step (400 bp) PCR result. Lanes 1-5: 10−1 to 10−5 dilution (first step); Lanes 6-10: 10−1 to 10−5 dilution (nested step).
J. Microbiol. Biotechnol. 2024;34:1146~1153 https://doi.org/10.4014/jmb.2401.01010
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