Journal of Microbiology and Biotechnology

eISSN 1738-8872
pISSN 1017-7825

Table. 1.

Table. 1. Table 1

Commonly used media for isolating gut microorganisms from human feces.

Medium	Carbohydrate	Extract and Enzymatic Hydrolysate	Vitamin	Blood	Rumen Fluid	SCFA	Antibiotic	Isolated Microbes (Genus)
5 µm-filtered BB	Glucose Sucrose	Yeast Extract Casein Enzymic Hydrolysate Papaic Digest of Soyabean Meal Animal Tissue Digest	B₆, K₃	-	-	-	-	Acidaminococcus, Actinomyces, Bacillus, Bacteroides, Bifidobacterium, Blautia, Citrobacter, Clostridium, Coprobacillus, Corynebacterium, Dietzia, Eggerthella, Enterococcus, Escherichia, Eubacterium, Facklamia, Flavonifractor, Fusobacterium, Klebsiella, Lysinibacillus, Parabacteroides, Pseudomonas, Ruminococcus, Streptococcus
BB with Blood	Glucose Sucrose	Yeast Extract Casein Enzymic Hydrolysate Papaic Digest of Soyabean Meal Animal Tissue Digest	B₆, K₃	Sheep Blood	-	-	-	Acidaminococcus, Alistipes, Anaerococcus, Bacteroides, Bifidobacterium, Blautia, Clostridium, Collinsella, Dietzia, Eggerthella, Enterococcus, Escherichia, Flavonifractor, Murdochiella, Odoribacter, Parabacteroides, Peptoniphilus, Pseudomonas, Staphylococcus
BB with Rumen Fluid	Glucose Sucrose	Yeast Extract Casein Enzymic Hydrolysate Papaic Digest of Soyabean Meal Animal Tissue Digest	B₆, K₃	-	+	-	-	Acidaminococcus, Alistipes, Bacteroides, Blautia, Campylobacter, Candida, Citrobacter, Clostridium, Dietzia, Eggerthella, Enterococcus, Escherichia, Facklamia, Flavonifractor, Klebsiella, Odoribacter, Parabacteroides, Pediococcus, Pseudomonas
TSB	Glucose	Pancreatic Digest of Casein Peptic Digest of Soybean	-	-	-	-	-	Bacillus, Corynebacterium, Dietzia, Enterococcus, Escherichia, Facklamia, Klebsiella, Lysinibacillus, Micrococcus, Pseudomonas, Staphylococcus, Streptococcus
TSB with Blood	Glucose	Pancreatic Digest of Casein Peptic Digest of Soybean	-	Sheep Blood	-	-	-	Acidaminococcus, Anaerococcus, Bacillus, Bacteroides, Blautia, Citrobacter, Clostridium, Collinsella, Dietzia, Eggerthella, Enterococcus, Escherichia, Eubacterium, Finegoldia, Flavonifractor, Klebsiella, Lactococcus, Lysinibacillus, Odoribacter, Parabacteroides, Peptoniphilus, Peptostreptococcus, Streptococcus
BHI	Glucose	Brain Heart Infusion Peptic Digest of Animal Tissue	-	-	-	-	-	Acidaminococcus, Ruminococcus
BHI with Colistin and Vancomycin	Glucose	Brain Heart Infusion Peptic Digest of Animal Tissue	-	-	-	-	Colistin Vancomycin	Bacteroides, Candida, Citrobacter, Clostridium, Dietzia, Enterococcus, Flavonifractor, Lactobacillus, Pseudomonas, Pediococcus
Gut Microbiota Medium	Glucose Fructose Maltose	Yeast Extract Meat Extract Tryptone Peptone	K₃, ATCC Vitamin Mix	-	-	Acetic Acid Isovaleric Acid Propionic Acid Butyric Acid	-	Bifidobacterium, Catenibacterium, Enterococcus, Lactobacillus
Modified Gifu Anaerobic Medium	Glucose Starch	Yeast Extract Meat Extract Liver Extract Peptone Soya Peptone Digested Serum	K₁	-	-	-	-	Acidaminococcus
Mueller Hinton Medium	Starch	Beef Extract Powder Acid Hydrolysate of Casein	-	-	-	-	-	Acidaminococcus, Ruminococcus
Phenylethyl Alcohol Medium with Blood	-	Papaic Digest of Soybean Meal Pancreatic Digest of Casein	-	Sheep Blood	-	-	β-Phenylethyl Alcohol	Acidaminococcus, Ruminococcus
BY Chocolate Medium	Starch	Yeast Extract Meat Extract Pancreatic Digest of Casein Acid Hydrolysate of Casein Peptic Digest of Animal Tissue	B1	Laky Horse Blood	-	-	-	Acidaminococcus, Clostridium, Ruminococcus
Potato Dextrose Medium	Glucose	Potato Infusion	-	-	-	-	-	Enterococcus, Megasphaera, Mitsuokella
Lactobacilli MRS Medium	Glucose	Yeast Extract Beef Extract Proteose Peptone (No.3)	-	-	-	Acetic Acid	-	Anaerostipes, Megasphaera, Mitsuokella
Mitis Salivarius Medium	Glucose Sucrose	Proteose Peptone (No.3+normal) Pancreatic Digest of Casein	-	-	-	-	Potassium tellurite	Bifidobacterium, Streptococcus
CIN Medium	Mannitol	Yeast Extract Beef Extract Pancreatic Digest of Gelatin Peptic digest of Animal Tissue	-	-	-	-	Sodium Deoxycholate Cefsulodin Novobiocin Triclosan	Fusobacterium
Listeria Enrichment Medium	Glucose	Yeast Extract Soy Peptone Pancreatic Digest of Casein	-	-	-	-	Acriflavine Cycloheximide Nalidixic acid	Eubacterium
CCFA Medium	Fructose	Peptic Digest of Animal Tissue	-	-	-	-	Cefoxitin Cycloserine	Coprobacillus
Tomato Juice Medium	-	Peptone Peptonized milk	-	-	-	-	-	Megasphaera, Mitsuokella
Drigalski Lactose Medium	Lactose	Pancreatic Digest of Casein Meat Peptic Digest Peptic Digest of Animal Tissue	-	-	-	-	-	Megasphaera, Mitsuokella
Selenite Medium	Lactose	Pancreatic Digest of Casein	-	-	-	-	-	Fusobacterium
Modified FM Medium	Glucose Starch	Yeast Extract Meat Extract Peptone Soy Peptone Liver Digest Serum Pancreatic Digest	-	-	-	-	Neomycin	Fusobacterium
DHL Medium	Sucrose Lactose	Meat Extract Peptone	-	-	-	-	Sodium Deoxycholate	Fusobacterium
Modified Peptone- Yeast Extract- Glucose Medium	Glucose	Yeast Extract Beef Extract Trypticase Peptone Peptone	K₁	-	-	Acetic acid	-	Faecalibacterium
Modified Bicarbonate- Buffered Medium	Glucose N-Acetylglucosamine	Soy Peptone	Vitamin Solution	-	+	-	-	Akkermansia

*SCFA, short-chain fatty acid; TSB, tryptic soy broth; BB, BD BACTEC Media; BHI, brain heart infusion

Data were collected from the references [17, 18, 21, 24].

J. Microbiol. Biotechnol. 2024;34:757~764 https://doi.org/10.4014/jmb.2311.11024