Fig. 2. Feasibility of the proposed approach.
(A) Fluorescence intensities of the FAM labeled H1 probe before and after assembly to hairpin structure. (B) SYBR Green I signals of the H1 probe during the self-primer elongation process. (C) FAM signals of the H2 probe during the self-primer elongation based signal recycling. “A” group, H2 probe; “B” group, H2 probe+ MRSA; “C” group, H2 probe+ MRSA+ DNA polymerase; “D” group, H2 probe+ MRSA+ DNA polymerase+ H1 probe; “E” group, H2 probe+ MRSA+ DNA polymerase+ H1 probe+ endonuclease. (D) Absorbance of the approach when essential components existed or not. “A” group, Blank control; “B” group, without MRSA; “C” group, without H1 probe; “D” group, without H2 probe; “E” group, without DNA polymerase; “F” group, without endonuclease; “G” group, with all.