Fig. 2.

Fig. 2. Topical administration of OSO improves AD symptoms. (A) Experimental procedure. (B) Representative photographs of mouse dorsal skin. (C) Histological examination of dorsal skin lesion epidermal thickness using H&E staining. Scale bar, 100 μm. (D) Number of infiltrated mast cells as determined using TB staining. Scale bar, 100 μm. (E) Dermatitis score was analyzed by summing the observed signs in the clinical trials, such as excoriation, scaling, edema, and erythema, with scores of 0 (almost clear), 1 (mild), 2 (moderate), and 3 (severe) for each sign. (F) Histological examination of dorsal skin lesion epidermal thickness was measured using H&E staining. Scale bar, 100 μm. (G) Number of infiltrated mast cells was measured using TB staining. Scale bar, 100 μm. (H) ELISA was performed to determine IgE levels in the serum. (I–J) At the end of the experiment, the mice were euthanized, and spleen weight (I) and inguinal lymph node length (J) were measured. The results are expressed as the mean ± SEM (n = 3–4 per group). *, p < 0.05; **, p < 0.01 compared with the OXZ-only group. $, p < 0.05; ^$$, p < 0.01 compared with the OXZ + SO group. ^##, p < 0.01 compared with the normal group (E-J).

© J. Microbiol. Biotechnol.