Fig. 4. 2 mM guaiacylglycerol-β-guaiacyl ether (GGE) was treated with no enzyme (blue), HeLac4c (red), and commercial TvLac (green) for testing biodegradation of laccase in pH 4 and analyzed with highperformance liquid chromatography (HPLC) without (A) and with addition of 1mM ABTS (B).
GGE retention time was approximately 18 minutes, and GGE polymers at 31 and 33 minutes were observed with the addition of 1 mM ABTS. GGE depolymerized unidentified fragmented GGE monomers at 11–14 min.