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Fig. 3.

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Fig. 3. Characterization of the secreted recombinant HeLac4c enzyme. Identification of protein N-glycosylation sites and copper binding sites of laccases expressed in S. cerevisiae (A). SDS-PAGE stained with Coomassie Brilliant Blue R-250 of HeLac4c with and without Endo H treatment for the removal of N-glycosylation (B). Effect of pH on oxidation activities of recombinant laccase (C) and stability of HeLac4c after pretreatment for 2 h at 10−80°C (D) Activity assays of recombinant HeLac4c enzyme were performed by oxidation of ABTS in 100 mM citrate-phosphate buffer at pH 4.0.
J. Microbiol. Biotechnol. 2024;34:930~939 https://doi.org/10.4014/jmb.2312.12043
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