Fig. 5.

Fig. 5. LE increased mitochondrial biogenesis through PGC-1α in obese sarcopenic mice. (A) Mitochondrial DNA (mtDNA) content in skeletal muscle of obese sarcopenic mice. (B) qRT-PCR analysis for the measurement of mRNA expression of mitochondria biogenesis regulated genes. (C) mRNA expressions of mitochondrial oxidative phosphorylation associated genes. (D) Western blot analysis for PGC-1α and AMPK phosphorylation (left panel) and their quantification (right panel) in skeletal muscle. Data are presented as mean ± standard error of the mean (SEM). *p < 0.05, **p < 0.01, and ***p < 0.001 versus HFD group. ND, normal diet fed group; HFD, high-fat diet fed group; HFD+LE, high-fat diet supplemented with 0.25% LE extract. Statistically significant differences were determined using one-way ANOVA followed by Tuckey’s post-hoc test.

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