Fig. 2. Lithospermum erythrorhizon (LE) prevents PA-induced muscle atrophy in C2C12 myotubes.
(A) Immunofluorescence staining for MHC 24 h after treatment. Scale bars represent 50 μm. (B) Quantification represents the average percent of fusion index. (C) RT-qPCR analysis of the mRNA levels of MAFbx and Murf1 in LE-treated C2C12 myotubes. (D) Immunoblotting for MHC-T, MHC-I, MHC-IIa, MHC-IIb, and β-actin of LE-treated C2C12 myotubes. Quantification represents the relation to NOR. All quantifications were performed in three independent experiments (n = 3). Error bars represent the standard deviation (SD). **p < 0.01 and ***p < 0.001 versus control (CON; PA treatment). Statistically significant differences were determined using one-way ANOVA followed by Tuckey’s post-hoc test.