Fig. 2.

Fig. 2. Phylogenetic tree of Bifidobacterium breve BS2-PB3. The comparisons of B. breve BS2-PB3 with other Bifidobacterium strains were carried out in the TYGS webserver (https://tygs.dsmz.de/) and were visualized by iTOL (https://itol.embl.de/). The tree was generated with TYGS, in which the MASH algorithm was used to compare B. breve BS2-PB3 with various strains of Bifidobacterium breve, Bifidobacterium animalis subspecies lactis, Bifidobacterium longum subspecies suillum, Bifidobacterium longum subspecies suis and Bifidobacterium longum subspecies infantis in the TYGS database. All pairwise comparisons were conducted using GBDP and accurate intergenomic distances were inferred under the algorithm 'trimming' and distance formula d5. The resulting intergenomic distances were used to infer a balanced minimum evolution tree with branch support via FASTME 2.1.6.1, including SPR postprocessing. Each branch support was inferred from 100 pseudo-bootstrap replicates. The tree was then visualized with iTOL.

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