N50 was related to the median and mean lengths of a set of sequences, in which it represented the length of the shortest read in the group of longest sequences, which together account for at least 50% of the nucleotides in the set of sequences. L50 was related to N50, indicating the number of sequences that, when arranged from longest to shortest, are needed to reach or exceed 50% of the total assembly size. bp, base pairs; GC, guanine-cytosine; CDS, coding sequence; RNA, ribonucleic acid; CRISPR, clustered regularly interspaced short palindromic repeats.