Fig. 4.

Fig. 4. In vitro cell-nanoparticle interaction and cytotoxicity. (A), (B) Cell viability assay with graded ChiNPs concentration; (C), (D), and (E) The in vitro cellular uptake of FITC-labelled ChiNPs (green) after 3 h. C) Dose-dependent efficiency of uptake using increasing concentrations of ChiNPs. D) The green dots indicate positive cell uptake. E) negative control without labeled ChiNPs. Cell membranes were labeled with Phalloidin-conjugated Alexa Fluor 680 (red) and nuclei with Hoechst 33342 (blue), scale bar = 100 μm, magnification 200X. Chart e) shows the data as mean ± standard deviation.

© J. Microbiol. Biotechnol.