Fig. 2. Enzyme linked oligonucleotide assay (ELONA) for the sensitivity assay of aptamer to PGP.
The purified PGP protein was diluted to the respective concentrations and dispensed into 96-well plates. DIG-labeled aptamer was added at each concentration and reacted at 4°C for 1 h. Panels A, 1 μg/ml of PGP; B, 5 μg/ml of PGP; C, 10 μg/ml of PGP.