Fig. 1. SDS-PAGE and modified-Western blot analysis of PGP specific aptamer.
Bacterial extracts of E. coli M15 and P. gingivalis were applied to SDS-PAGE and their proteins were transferred to PVDF membranes for the Western blot analysis. Panels A, SDS-PAGE; B, modified Western blot analysis with 1 pM aptamer; C, with 0.1 pM aptamer. Lanes M, Molecular weigh marker (kDa); 1, the bacterial extract of E. coli M15; 2, the bacterial extract of E. coli M15 (pQE-PGP) induced with 1 mM IPTG; 3, partially purified PGP; 4, the bacterial extract of P. gingivalis.