Fig. 4.

Fig. 4. Validation of RNA-Seq results using RT-qPCR. Relative expression levels of (A) cytokines including IL-18, IL-27, LIF, IFN-β, and TNF-α (B) chemokines including CCL2, CCL4, CXCL2, CXCL3, CXCL10, and CXCL11 (C) immune regulatory factors including CD40 and ICAM, as determined by RT-qPCR. (D) Comparison of log2 fold changes obtained from RNA-Seq and RT-qPCR for selected genes. (E) Linear regression analysis of X-axis (log2 fold change qPCR) and Y-axis (log2 fold change RNA-seq) data showed a correlation coefficient (R) of 0.79. Data are presented as mean ± SEM. Statistical analysis was performed using separate tests for each gene, and the significance levels were indicated as follows: **p < 0.01, ***p < 0.001 compared with the CON group.

© J. Microbiol. Biotechnol.