Fig. 5. Effects of PDE on p65 translocation in RAW 264.7 cells.
RAW 264.7 cells were treated with PDE at 25, 50, and 100 μg/ml for 30 min. (A) PDE induced p65 NF-κB nuclear translocation in RAW 264.7 cells. (B) Quantification of nuclear p65 and cytosolic p65. The expressions of p65, Lamin B1, and α/β-Tubulin was detected by western blot. RAW 264.7 cells were treated with PDE at 50, and 100 μg/ml for 30 min. (C) Immunofluorescence. The data represent the mean ± SD (n = 3). **p < 0.01 and ***p < 0.001 vs. control group. PDE, Pinus densiflora pollen extract; Cont, control; MitoSOX, mitochondrial superoxide; DAPI, 4',6-diamidino-2-phenylindole.