JMB

eISSN 1738-8872
pISSN 1017-7825

Fig. 5.

Download original image
Fig. 5. Effects of PDE on p65 translocation in RAW 264.7 cells. RAW 264.7 cells were treated with PDE at 25, 50, and 100 μg/ml for 30 min. (A) PDE induced p65 NF-κB nuclear translocation in RAW 264.7 cells. (B) Quantification of nuclear p65 and cytosolic p65. The expressions of p65, Lamin B1, and α/β-Tubulin was detected by western blot. RAW 264.7 cells were treated with PDE at 50, and 100 μg/ml for 30 min. (C) Immunofluorescence. The data represent the mean ± SD (n = 3). **p < 0.01 and ***p < 0.001 vs. control group. PDE, Pinus densiflora pollen extract; Cont, control; MitoSOX, mitochondrial superoxide; DAPI, 4',6-diamidino-2-phenylindole.
J. Microbiol. Biotechnol. 2024;34:644~653 https://doi.org/10.4014/jmb.2309.09026
© J. Microbiol. Biotechnol.