Fig. 7. The protective ability of anthocyanins against ROS generation and apoptosis caused by H2O2 treatment was offset by ZnPP in ARPE-19 cells.
Cells were treated with anthocyanins and/or ZnPP for 1 h and then further treated with H2O2 for 24 h. (A and B) Representative flow cytometry results (A) and their average values (B) according to DCF-DA staining. (C and D) Representative histograms (C) and quantitative results (D) of flow cytometry analysis following double staining of annexin V and PI. (E) Cell viability was assessed using MTT assay.