Fig. 5. Anthocyanins attenuated ROS production and reduced GSH/GSSG ratio in ARPE-19 cells under H2O2-treated conditions.
Cells exposed with or without anthocyanins for 1 h were stimulated with H2O2 for 1 h (A, B and C) or 24 h (D). (A and B) Representative histograms of flow cytometry (A) and the frequency of DCF-positive cells (B). (C) Representative fluorescence images of ROS production. (D) Bar chart indicated the GSH/GSSG ratio following the exposure to H2O2 and pretreatment with anthocyanins.