Fig. 4. Anthocyanins suppressed H2O2-induced mitochondrial impairment in ARPE-19 cells.
Cells exposed with or without anthocyanins for 1 h were treated with H2O2 for 24 h. (A and B) Representative histograms (A) and JC-1 monomer ratios (B) of flow cytometry using JC-1 staining in each treatment group. (C and D) The expression of cytochrome c in the mitochondrial and cytosolic fractions was investigated by immunoblotting.