Fig. 3.

Fig. 3. Anthocyanins ameliorated H₂O₂-induced apoptosis in ARPE-19 cells. Cells were exposed to anthocyanins for 1 h prior to treatment with H₂O₂ for 24 h. (A and B) Representative histograms (A) and quantitative results (B) of flow cytometry analysis by Annexin V/PI staining. (C and D) Images of representative nuclei (C, 400×) and results of quantitative analysis obtained after DAPI staining. (E) Expression changes of the indicated proteins obtained through immunoblotting. (F) Differences in caspase-3 activity by treatment group.

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