Fig. 4.

Fig. 4. E484K and K417N demonstrated a synergistic effect on antibody escape. Pseudoviruses, which express spike proteins harboring the following mutations: D614G or D614G-N501Y (black lines), D614G-E84K or D614G-K417N (blue lines), D614G-E484K-K417N or D614G-N501Y-E484K-K417N (red lines), were mixed with Casirivimab at 37°C for 1 h before infection into Hcc15-hACE2. At 72 h post-transfection, luciferase activities were measured and normalized to those of pseudoviruses that express spike proteins harboring D614G mutation. One representative data from two independent experiments is shown (top panel). IC50 was calculated by polynomial regression as described in the Materials and Methods and plotted (bottom panel). *, p < 0.05; **, p < 0.02; ***, p < 0.001.

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