Fig. 7. Effects of EHE on lipid accumulation and adipogenic transcription factors in liver tissue of HFD-fed mice.
(A) Representative liver sections stained with H&E (200× magnification). (B) Representative liver sections stained with Oil Red O (200× magnification). (C) Western blot shows PPAR-γ, SREBP-1c, and C/EBP-α protein expression in liver tissues. β-actin served as the loading control. Relative protein expression levels, quantified as the band intensity relative to the intensity of the β-actin band, are shown for PPAR-γ, SREBP-1c, and C/EBP-α. All data are expressed as the mean ± SD of the experiment. ##p < 0.01 compared to the LFD group; **p < 0.01 compared to the HFD group.